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KMID : 0942820040030020088
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Journal of Korean Brain Tumor Society
2004 Volume.3 No. 2 p.88 ~ p.94
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Isolation and Characterization of Differentially Expressed Genes in Human Glioblastoma using Suppression Subtractive Hybridization
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Yu Na-Mi
Ahn Jung-Yong
Kim Kye-Seong
Kim Chang-Hyun
Kim Tai-Gyu
Choi Eun-Jin
Kim Jin-Kyeoung
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Abstract
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A variety of genetic alterations in human glioblastomas comprises signal transduction and cell cycle arrest control of cellular processes. Subtractive hybridization is potentially a faster method for identifying differentially expressed genes associated with a particular disease state. Using the technique of subtraction, we isolated a novel gene that is overexpressed in glioblastoma as compared to normal brain tissue. Glioblastoma was used as tester and brain normal was used as driver. We identified 23 novel genes by BLAST of the digested 130 clones. In this study, we selected that this 571 bp cDNA of “clone 25” was not homologous to any of the known genes in the Genbank database. The expressions of mRNA of “clone 25” of brain tumors were significantly higher than normal brain tissue. “Clone 25” is clearly more frequently and more strongly expressed in several carcinoma cell lines compared to corresponding normal tissues of lung, colon, and prostate. To test the time course for G0-phase arrest, serum stimulation and expression at various times for RT-PCR was performed. We confirmed the mRNA expression of “clone25” was up-regulation for 0.5, 1, 2, and 4hr of WI-38 cell differentiation.
The novel gene, “clone 25” was upregulated in glioblastoma tissue and several cancer cell lines. This gene is time dependent activation during time course of serum stimulation. Expression and activation of “clone 25” may be important and specific development roles of glioblastoma.
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KEYWORD
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Genes, Glioblastoma, Tumorigenesis, Suppression subtractive hybridization
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